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Image Search Results
Journal: Molecular and cellular endocrinology
Article Title: Estrogen receptor beta maintains expression of KLF15 to prevent cardiac myocyte hypertrophy in female rodents
doi: 10.1016/j.mce.2017.11.004
Figure Lengend Snippet: (a) flow cytometry analysis of ACTA2 (top) and MYH7 (bottom) expressions, bar graph data are from 3 exps. *p < 0.05 vs. control, +p < 0.05 for ANGII versus same + ß-LGND; (b) KLF15 protein and mRNA (c) were significantly stimulated by AngII that was augmented by KLF15siRNA, analyzed by western blot and real time PCR, respectively.
Article Snippet: Additional antibodies and phospho-specific antibodies used for immuno-blots were obtained from the followings: Cell Signaling Technology (Danvers, MA) TAK1 (D94D7) (#5206), Phospho-ATF-2 (Thr71) (#9221), Phospho-TAK1 (Thr187) (#4536); Santa Cruz, Biotechnology (Dallas, TX), KLF15 (A5) (SC-271675), GAPDH (0411) (sc-47724), MYH7 (A4.951) (sc-53090), Actin (2Q1055) (sc-58673), p38 Antibody (A-20) (sc-535), phospho-p38 (Thr 180/Tyr 182) (sc-17852-R); (
Techniques: Flow Cytometry, Western Blot, Real-time Polymerase Chain Reaction
Journal: Neural Regeneration Research
Article Title: The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia
doi: 10.4103/1673-5374.165512
Figure Lengend Snippet: Immunoreactivity and mRNA expression of alpha-smooth muscle actin (α-SMA) in the frontal cortex of rats with chronic cerebral ischemia and the effects of fasudil treatment. (A) Left: Representative photomicrographs of α-SMA-immunoreactive cells (200×). Right: Quantification of α-SMA immunoreactivity. Immunoreactivity of α-SMA was determined at 3, 6, and 9 weeks (wk) after surgery. Arrows show α-SMA-immunoreactive cells. (B) mRNA expression of α-SMA determined by RT-PCR in the three [sham-operated (sham), ischemia, fasudil] groups at 3, 6, 9 wk after surgery. The mRNA results are expressed as the ratio of the target gene band intensity to that for α-actin. There were 10 rats at every time point per group. Data are presented as the mean ± SD and analyzed using one-way analysis of variance and the Student's t -test. * P < 0.05, vs . sham group; # P < 0.05, vs . ischemia group.
Article Snippet: Overnight incubation with rabbit anti-microtubule-associated protein 2 (MAP2) polyclonal antibody (1:100;
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction